Syrian Foreign Minister Walid Muallem has said he rejects "utterly and completely" that Syrian government forces used chemical weapons.
He was speaking in Damascus after the US said there was "undeniable" proof of a chemical attack.
He said UN weapons inspectors had been unable to go to a second site because rebels could not ensure their safety.
The US and its allies are considering launching strikes on Syria in response to deadly attacks last week.
Mr Muallem told a news conference that if a military act was carried out against Syria, the pretext of chemical weapons would be false, baseless and groundless.
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