Twitter reported a net loss of $125m (£82m) in the fourth-quarter, beating analyst expectations.
It also said revenue grew faster than expected, increasing by 95% to $479m during the October to December period.
Total monthly active users were 288 million, an increase of 20% from the year earlier.
However, growth from last quarter was significantly slower: the site managed to add only four million users in the past three months.
Twitter tried to explain away the slowing growth, saying it lost approximately four million users during the period as a result of integrating various third-party applications.